The purpose of this research is to examine fastness and dimensional stability of the knitted fabrics made of three types of new generation regenerated cellulose fibres such as Naia™ Renew, Tencel™ Lyocell and Ecocell™. The perspiration, washing, water and rubbing fastness tests and also dimensional stability determination were performed. The data was compared with the ones of the cotton fabric. The goal of this study is comparing whether recently developed new generation regenerated cellulose fibers compared to cotton, presents better performance considering customer perceptions. Accordingly, the acidic and basic perspiration (TS EN ISO 105–E04), washing (TS EN ISO 105–C06), water (TS EN ISO 105–E01) and rubbing fastness (TS EN ISO 105–X12) tests and also dimensional stability (TS EN ISO 3759) determination was performed in this concept.