This research was conducted so as to find out whether measurement invariance is established for the gender inequality subscale in the World Values Survey among the subsamples of Turkey, Great Britain and the Netherlands, and whether the items in this subscale show differential item functioning among these three samples. The sample includes three subsamples, namely Turkish, British and Dutch samples, which participated in the seventh cycle of the World Values Survey. The gender inequality subscale of the World Values Survey is the measure of this research. So as to test the dimensionality of the relevant subscale, confirmatory factor analysis was conducted and the uni-dimensional structure of the subscale was confirmed. In order to reveal whether the gender inequality subscale shows measurement invariance among subsamples, multi-group confirmatory factor analysis was conducted and measurement invariance was established at configural level for Turkish-British samples while metric invariance was established both for Turkish-Dutch samples and and Dutch-British samples. According to the findings of the SIBTEST and likelihood ratio test, which were carried out to reveal whether the items in the gender inequality subscale operate differentially among these three samples; it has been revealed that two items operate differentially across Turkish and British samples; two items operate differentially across Turkish and Dutch samples, and finally five items operate differentially between Dutch and British samples. According to these results, it might be suggested to be more careful while making cross-cultural comparisons among these samples based on the gender inequality subscale in the World Values Survey.