For production of active proteins using heterologous expression systems, refolding of proteins from inclusion bodies often creates a bottleneck due to its poor yield. In this study, we show that molecularly imprinted polymer (MIP) toward native lysozyme promotes the folding of chemically denatured lysozyme. The MIP, which was prepared with 1 M acrylamide, 1 M methacrylic acid, 1 M 2-(dimethylamino)ethyl methacrylate, and 5 mg/mL lysozyme, successfully promoted the refolding of lysozyme, whereas the non-imprinted polymer did not. The refolding yield of 90% was achieved when 15 mg of the MIP was added to 0.3 mg of the unfolded lysozyme. The parallel relationship between the refolding yield and the binding capacity of the MIP suggests that MIP promotes refolding through shifting the folding equilibrium toward the native form by binding the refolded protein.

a 現） 山梨大学医学部附属病院薬剤部 b 現） 国際医療福祉大学薬学部 医療薬学 40(11) 672-676 (2014) カラゲニン足蹠浮腫モデルを用いた 各種ケトプロフェンテープ剤の抗炎症効果比較 Received July 10, 2014 Accepted September 18, 2014 Differences in the anti-inflammatory effects induced by brand-name and generic ketoprofen tapes have not yet been examined. The aim of the present study was to investigate the anti-inflammatory effects of 9 ketoprofen tapes on rat carrageenan-induced paw edema and also determine the amount of ketoprofen released from these tapes. The suppression of carrageenan-induced paw edema more than 3 hours after the tapes had been applied was significantly stronger with the 4 ketoprofen tapes tested than with the control. The brand-name product released the highest amount of ketoprofen 3 hours after the tapes had been applied. The amount of ketoprofen released from the brandname product was higher than that by 4 out of the 8 generic products tested. Furthermore, a correlation was found between anti-inflammatory effects and the amount of ketoprofen released 3 hours after applying the ketoprofen tapes (r = 0.552). Thus, the biological equivalents of generic products should be considered because the anti-inflammatory effects and amount of ketoprofen released differed between the brand-name and generic ketoprofen tapes.