A field experiment was conducted to evaluate the effect of Azotobacter and L-tryptophan (L-TRP) application on the growth, yield and nitrogen content of wheat (Triticum aestivum L.) crop. Two levels of L-TRP (10G 3 and 10G 4 M) were tested with and without Azotobacter inoculation in a fertilized (NPK: 125-100-60 kg haG 1 , respectively) field. Results revealed that Azotobacter inoculation and L-TRP application significantly affected the wheat crop, however, their combined application produced more pronounced effect as compared with their separate application. Combined application of Azotobacter and 10G 3 M L-TRP significantly increased the grain yield (21.3%), straw yield (20.7%), plant height (5.8%), number of tillers (15.3%), number of spikelets spikeG 1 , (12.3%), spike length (11.6%), 1000-grain weight (6.4%), nitrogen concentration in grains (31.4%) and straw (26.1%) and total nitrogen uptake (56.3%), compared with untreated control.