For the first time, a novel EpCAM aptamer (SYL3C)-DIBO-AF594 fluorescent conjugate was synthesised by bioorthogonal chemistry utilizing a strain promoted alkyne-azide cycloaddition (copper free click) reaction (SPAAC). The ligation efficiency of SPAAC was improved by freeze-thaw cycles. The obtained conjugate showed target specific binding and aided in the imaging of various EpCAM positive cancer cell lines like MCF7, MDAMB453, Weri-RB1 and PC3.

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The mixture of applications increases and supported over optical networks, to the network customers new service guarantees must be offered .The partitioning the data into multiple segments which can be processed independently the useful data to be transferred before a predefined deadline .this is a deadline driven request. To provide the request the customer chooses the bandwidth DDRs provide scheduling flexibility for the service providers. It chooses bandwidth while achieving two objectives 1.satisfying the guaranteed deadline 2.decreasing network resource utilization .by using bandwidth allocation policies improve the network performance and by using mixed integer linear program allows choosing flexible transmission rates.