In order to verify the reliability of hybrid population issued from crossing between 3 clones of "Picholine marocaine" cultivar and the "Picholine du Languedoc" cultivar, the descendants and their parents were analysed using 35 microsatellite loci. No offspring resulted from self crossing of "Picholine marocaine" cultivar and 218 descendants among 220 analysed are legitimate. This study showed clearly a segregating population and may be used as a genetic material for linkage map construction and for phenotyping resistance traits related to Spilocaea oleagina disease.
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