Operation of nuclear facilities such as research reactor and it's supporting installation in Serpong Nuclear Area may release controlled radionuclides to Cisadane River and then it would flow to Jakarta Bay. There are limited marine radioecology studies or radionuclides monitoring at Jakarta Bay. Therefore monitoring of 239/240 Pu and 137 Cs was carried out from Tanjung Pasir to Tanjung Kerawang. The ERICA Assessment Tool was used to evaluate radiological risk in the marine environment of Jakarta Bay area. The 137 Cs concentration in sea water and sea sediments were in range of 0.17-1.17 Bq.m-3 and 0.34-1.21 Bq.kg-1 , respectively. Moreover, result of 239/240 Pu measurement showed that concentration at sea water and sea sediment were range from < MDA-0.53 mBq.m-3 and 2.64-55.70 mBq.kg-1 respectively. The results of risk analysis were indicated that all the total dose rates per organism were millions time lower than the screening rate (10 mGy.h-1).

2-Hexenal is an alpha,beta-unsaturated carbonyl compound which forms cyclic 1,N2-propano adducts in vitro. The adduct formation in vivo was not reported by others to date. Because this type of adduct is considered promutagenic (2-hexenal is actually mutagenic and genotoxic) and humans are permanently exposed to this compound via vegetarian food, 2-hexenal may play a role in carcinogenicity. To improve the cancer risk assessment, we developed a new 32P-postlabeling technique for this compound and optimized the different steps of the postlabeling procedure. The results of the postlabeling methods are shown. A labeling efficiency of 35%, a recovery of 10% for the synthesized standards, and a detection limit of three 2-hexenal adducts per 10(8) nucleotides was achieved. After gavage of 500 mg/kg of body weight to male Fischer 344 rats, the respective DNA adducts were detected in rat liver DNA. With this study, we demonstrate in vivo adduct formation of 2-hexenal for the first time. Highest adduct levels were found 2 days after gavage, and after 4 days, the level was even higher than after 1 day. No adducts were detected 8 h after gavage. The respective adducts could not be found as a background in tissues of untreated rats or in calf thymus DNA at the limit of detection.

No abstract

No abstract

No abstract