gene reduces the expression of the kafirin and ASG genes would be valuable information to better understand the linkage between the soft-kernel characteristics and the improved protein quality of the P7210 line and of the higher yielding lines derived from its crosses with elite inbreds.
Investigations were performed to study the chemical form in which nickel and cobalt are transported in sieve tubes. Ricinus communis plants were used since they allow easy collection of relatively pure phloem sap. In the experiments labelled nickel or cobalt were given to the nutrient solution. The tapped phloem exudate was separated by gel filtration on Sephadex or by paper electrophoresis. Nickel and cobalt were bound to complexing organic compounds with a molecular weight in the range of 1000—5000. The overall charge of the nickel complex was negative, and the bulk of cobalt also appeared to complex to a compound with negative overall charge.
The extent of redistribution in apple was described by calculating the fruit/leaf ratio of the cation content. It was found that the redistribution diminishes in the sequence K > Mg > Ca ∼ Mn. These results are more or less in agreement with those found elsewhere.
Investigations were made to see whether it was possible to account for the differences in redistribution by the phloem by means of the solubility of these cations in the sieve tube sap. As model plants Yucca flaccida and Ricinus communis were used, plants from which it was possible to obtain phloem sap in a rather pure state. It was found that the addition of potassium and magnesium as a chloride in the usual investigated range of concentrations did not give precipitation. With calcium and manganese, however, a precipitate soon occurred. Manganese was demonstrated to be less soluble than calcium. For Ricinus the maximum amount of calcium and manganese the sieve tube sap could contain before precipitation set in was higher than for Yucca sap.
The results confirm the possibility that the redistribution of the different cations in the plant can be related inter alia to their solubility in the sieve tube sap.
It was also found that the calcium in the phloem sap is present in ionic condition. Thus the normal laws of solubility should be applicable.
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