Ascochitine production by 24 isolates of three fungal species causing the Ascochyta disease complex in pea plants, was examined. All eight isolates of Ascochyta pisi Lib. secreted ascochitine to liquid nutrient medium in amounts of 0.1 to 1.2 mg/l. However, no ascochitine was found in culture filtrates of Mycosphaerella pinodes (Berk. and Blox.) Vestergr. or of Phoma medicaginis var. pinodella (Jon.) Boerema although the aggressiveness of these two species as pathogens was shown to be higher than that of A. pisi. Individual isolates of each of the three fungal species examined did not differ in their pathogenicity.

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