This study aimed to investigate the effect of MOl or MSC supplementation on some blood constituents, semen characteristics, testis and epididymis measurements in Barki rams. Fifteen Barki rams aged 10-12 months divided into three similar groups (5each). Rams in G1 were fed the basal diet supplemented by MOL (5% of CFM), rams in G2 fed the basal diet supplemented by MSC (10% of CFM) while rams in G3 (control) were fed the basal diet without supplements. Blood samples were collected and erythrocytes, Hb, PLT, PCV, MCV, MCH, MCHC, ALT, AST, glucose, uric acid, creatinine and testosterone were determined. Semen was collected and semen physical characteristics as well as of the epididymis and testes biometric were evaluated. Results indicated that MOL and MSC supplementation to ram rations significantly affected glucose, creatinine, urea, ALT, AST and testosterone levels, and insignificantly on erythrocyte, Hb, PLT, PCV, MCV, MCH, and MCHC. In this regard, MOL supplementation to ram rations increased MCV, PLT and decreased RBCs, Hg, MCH, MCHC, and PCV; while, MSC supplementation increased RBCs, Hg, MCHC, PLT, and urea. The addition of MOL or MSC increased ALT, AST, and creatinine, and decreased testosterone and glucose levels. Both supplementation (MOL or MSC) in Barki rams' feed improved most characteristics of semen such as volume, motility, vitality and normality, and reduced mortality and abnormalities and there is no significant effect on testes measurements. The highest testicular weight and width were observed in the MOL group. The testicular size was relatively higher for rams fed a moringa-free diet compared to those supplemented with MOL or MSC. It could be concluded that supplementation of MOL and MSC rations into Barki rams' rations improved most of the semen characteristics (volume, motility, vitality and normality) and reduced mortality and malformations. However, it negatively affects kidney function (increased urea and creatinine) and liver enzyme activities (ALT & AST).
This study sought to study the effect of certain strains of fungi (Trichoderma harzianum) and yeast (Saccharomyces cerevisiae D-47) inoculation on silage chemical composition, fermentation characteristics and in vitro digestibility. Four treatments were tested i.e., control (C): corn silage without inoculants, Y: corn silage applied with saccharomyces cerevisiae D-47, T: corn silage applied with Trichoderma harzianum and Y+T: corn silage applied with both inoculants. Chopped whole corn were moved and sealed in polyethylene silo bags (1.5 to 2 kg) using a vacuum sealer for air removal. The silo bags were stored at room temperature for different ensiling times (zero time, 5 h, 10 h, 20 h, and 2, 4, 8, 14, 25 and 35 days). Obtained results showed that inoculants had no significant effect on DM and OM, while ensiling time significantly (P<0.04) decreased DM and OM content from 32.78% and 94.12% to 31.91% and 93.23% at 35d of ensiling respectively. treatments and ensiling time significantly (P< 0.5) increased CP and NFE in corn silage than the control (c) group, while CF, NDF and ADF significantly decreased, along with time of ensiling. Values of pH along with time of ensiling gradually decreased from 6.01 at zero time to 3.9 at 35d. Ammonia-N decreased significantly (P<0.001) from 39.06 in control comparing to 37.95, 38.44 and 37.0 g/kg total N. Ammonia nitrogen gradually increased significant (P<0.001) with the time of ensiling in a curve-linear way. Treating silages with inoculant yeast (Y), trichoderma (T) or both (Y+T) led to an increase (P<0.001) in lactic acid concentration being 39.5 and 38.99 g/kg DM, respectively. The best value was that of Y+T treatment (40.77g/kg DM), acetic and butyric acid followed opposite trend. Time of ensiling negatively correlated with concentration of both formic and citric acids while it was positively correlated with the concentration of succinic acids. Treating silages with inoculant increased total bacteria (5.51, 7.69, 7.69 and 7.81 log10 cfu/g DM) for the control, Y, T and Y+T, respectively. Similarly treating silages with inoculant significantly increased lactic acid bacteria (6.46, 6.89, 6.97 and 7.03 log10 cfu/g DM) for the control, Y, T and Y+T, respectively. Results of total yeasts (log10 cfu/g DM) followed the same pattern being less for control (5.45) and increased with the inoculant treatment being 6.57, 6.44 and 6.89 for the same respective order. Inoculants increased both IVDMD and IVOMD significantly; the best values were that of corn silage inoculated with both yeast + Trichoderma.
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