Biofilms are communities of sessile microbes that are phenotypically distinct from their genetically identical, free-swimming counterparts. Biofilms initiate when bacteria attach to a solid surface. Attachment triggers intracellular signaling to change gene expression from the planktonic to the biofilm phenotype. For , it has long been known that intracellular levels of the signal cyclic-di-GMP increase upon surface adhesion and that this is required to begin biofilm development. However, what cue is sensed to notify bacteria that they are attached to the surface has not been known. Here, we show that mechanical shear acts as a cue for surface adhesion and activates cyclic-di-GMP signaling. The magnitude of the shear force, and thereby the corresponding activation of cyclic-di-GMP signaling, can be adjusted both by varying the strength of the adhesion that binds bacteria to the surface and by varying the rate of fluid flow over surface-bound bacteria. We show that the envelope protein PilY1 and functional type IV pili are required mechanosensory elements. An analytic model that accounts for the feedback between mechanosensors, cyclic-di-GMP signaling, and production of adhesive polysaccharides describes our data well.

ABSTRACT. This paper presents a summary of the first 10 years of operating the Hobby-Eberly Telescope (HET) in queue mode. The scheduling can be quite complex but has worked effectively for obtaining the most science possible with this uniquely designed telescope. The queue must handle dozens of separate scientific programs, the involvement of a number of institutions with individual Telescope Allocation Committees, as well as engineering and instrument commissioning. We have continuously revised our queue operations as we have learned from experience. The flexibility of the queue and the simultaneous availability of three instruments, along with a staff trained for all aspects of telescope and instrumentation operation, have allowed optimum use to be made of variable weather conditions and have proven to be especially effective at accommodating targets of opportunity and engineering tasks. In this paper, we review the methodology of the HET queue, along with its strengths and weaknesses.

We report the first detection of a planetary-mass companion to a star using the High Resolution Spectrograph (HRS) of the Hobby-Eberly Telescope (HET). The HET-HRS now gives routine radial velocity precision of 2-3 m s −1 for high SNR observations of quiescent stars. The planetary-mass companion to the metal-rich K0V star HD 37605 has an orbital period of 54.23 days, an orbital eccentricity of 0.737, and a minimum mass of 2.84 Jupiter masses. The queue-scheduled operation of the Hobby-Eberly Telescope enabled us to discovery of this relatively short-period planet with a total observation time span of just two orbital periods. The ability of queue-scheduled large-aperture telescopes to respond quickly to interesting and important results demonstrates the power of this new approach in searching for extra-solar planets as well as in other ares of research requiring rapid response time critical observations.

No abstract

This paper describes spectra of quasar candidates acquired during the commissioning phase of the Low-Resolution Spectrograph of the Hobby-Eberly Telescope. The objects were identified as possible quasars from multicolor image data from the Sloan Digital Sky Survey. The ten sources had typical r ′ magnitudes of 19-20, except for one extremely red object with r ′ of ≈ 23. The data, obtained with exposure times between 10 and 25 minutes, reveal that the spectra of four candidates are essentially featureless and are not quasars, five are quasars with redshifts between 2.92 and 4.15 (including one Broad Absorption Line quasar), and the red source is a very late M star or early L dwarf.