Podophyllotoxin finds a lot of scopes in pharmaceutical field as it show wide biological activities. Several analogues of podophyllotoxin were reported variety of biological activities such as cathartic, cytotoxic, antimitotic, anticancer, antitropical skin disease, antimalarial, virucidal, fungicidal etc. Experiment was carried out to synthesize 6, 6a-dihydro-2, 3-dimethoxy-9-methyl-11bH benzo [c] fluoren - 5, 7 dione and Synthesis of 6, 6a-dihydro-3-methoxy-2, 9-dimethyl-11 bH benzo [c] fluoren - 5, 7 dione. The products were re-crystallized in ethanol and characterized by spectrometric methods, IR, NMR and Mass spectroscopy. A yield of 82.72 and 83.6 percent were recorded respectively. The anti-mitotic activities of the synthesized analogues were examined by the onion root tip method with reference to b- apopicropodophyllin and Podophyllotoxin. In the present work, the synthetic analogues D and E have shown increased anti-mitotic activities with ID50 of 2.005 x 10-6 M and 2.260 x 10-6M respectively with reference to standard and control