The physical and nutritional requirements for growth of Hexamita inflata have been studied in axenic cultures. The flagellate was capable of growing over a wide range of temperature (5 °C to 25 °C), of hydrogen ion concentration (pH 4.5 to 8.5), and of salinity (3 to 28‰); and required a reducing or anaerobic environment. The requirement of an egg-yolk suspension for growth was partially satisfied by unsaturated fatty acids. Attempts to replace the peptone by mixtures of amino acids were not successful. A simple medium containing a vitamin mixture, linoleic acid, glucose, cysteine, peptone, and salt has been formulated.

Hexamita inflata in axenic culture by serial transfer in a beef serum or egg yolk enriched medium containing antibiotics.An examination of the urine of hamsters bearing adenovirus-12 induced tumors showed an elevated excretion of methylated purines. The weight of the animals increased 1 to 5% while the purines excreted increased 200%.