This article presents an application of the digital imaging in the reading of a slide in optical microscopy, by applying a photometric correction of images issued out of the microscope, an interpolation of images captured by a CDD sensor (decoding) and the implementation of a mosaicking in order to obtain a virtual slide of big size. This study is particularly decisive in a context of telediagnosis applications in optical microscopy, using this type of sensor and manipulating images of large dimensions. We also propose some solutions which improve the efficiency of the telediagnosis chain, and the quality of the visualized images.