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During aging, hematopoietic stem cell (HSC) function wanes with important biological and clinical implications for benign and malignant hematology, and other co-morbidities, such as cardiovascular disease. However, the molecular mechanisms regulating HSC aging remain incompletely defined. GATA2 haploinsufficiency driven clinical syndromes initially result in primary immunodeficiencies and routinely evolve into hematologic malignancies on acquisition of further epigenetic mutations in both young and older patients. Using a conditional mouse model of Gata2 haploinsufficiency, we discover that during aging Gata2 promotes HSC proliferation, monocytosis, and loss of the common lymphoid progenitor. Aging of Gata2 haploinsufficient mice also offsets enhanced HSC apoptosis and decreased granulocyte-macrophage progenitor number normally observed in young Gata2 haploinsufficient mice. Transplantation of elderly Gata2 haploinsufficient HSCs impairs HSC function with evidence of myeloid bias. Our data demonstrate that Gata2 regulates HSC aging and suggest the mechanisms by which Gata2 mediated HSC aging has an impact on the evolution of malignancies in GATA2 haploinsufficiency syndromes.

Objective: Photon-induced photoacoustic streaming (PIPS) with pineapple peel extract (PPE) and ethylenediaminetetraacetic acid (EDTA) as a final endodontic irrigant on the push-out bond strength (PBS) of root filling material to conventional irrigation NaOCl with EDTA. Methods: An in vitro study at Dar Al Uloom University was conducted over three months. Root canal preparation was performed. Based on final irrigation, all the specimens were divided randomly into four groups (n=10) Group 1: 2.25% NaOCl+ 17% EDTA (control), Group-2: 2.25% NaOCl+PIPS + 6.25% PPE, Group-3: 2.25% NaOCl+PIPS + 17% EDTA, Group-4: 2.25% NaOCl + 6.25% PPE. Canals were obturated and sealed with AH Plus sealer. Root sectioning was performed at 1mm thickness and PBS testing was performed using the universal testing machine. The debonded samples were analyzed for failure mode. ANOVA compared the means and standard deviations (SD) of all investigated group. Assessment of multiple comparisons was performed using Tukey’s post hoc test. Results: The maximum PBS was demonstrated by the coronal section of Group-2 (2.25%NaOCl +PIPS+6.25%PPE) specimens (8.21±0.81MPa). The apical section of Group-1 (2.25% NaOCl+17% EDTA) specimens demonstrated minimum bond strength (2.80±0.18 MPa). The intergroup comparison revealed that Group-3 (2.25% NaOCl +PIPS +17% EDTA) and Group-2 demonstrated comparable outcomes (p>0.05). Group-4 (2.25% NaOCl +6.25% PPE) specimens established significantly lower values than Group-3 and Group-4 (p<0.05). Conclusion: Photon-induced photoacoustic streaming with pineapple peel extract and EDTA demonstrated better bond strength of root canal sealer and have the potential to be used as the final irrigant. doi: https://doi.org/10.12669/pjms.39.4.7370 How to cite this: AlGhamdi ARS, Alotaibi B, Alsalhi H, Aljuailan RI, Barakat A. Photo-induced photoacoustic streaming and pineapple peel extract as final irrigant on extrusion bond strength of root filling material to canal wall . Pak J Med Sci. 2023;39(4):---------.  doi: https://doi.org/10.12669/pjms.39.4.7370 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Push-out bond strength (POBS) and failure analysis of gutta-percha and Martens hardness (MH) of canal dentin sealed with Bio-ceramic sealer (BCS). Specimens underwent endodontic treatment. Based on the method of disinfectants, specimens were classified into 4 groups. Group 1:5.25% NaOCl+Q-mix, group 2:ECYL+Q-mix, group 3:LGE+Q-mix, and group 4:RBP+Q-mix. Before obturation MH was assessed using a microhardness tester. Canals were obturated, and sealed with Bio-Root RCS. Each root was cut horizontally in 2 mm thickness and placed under the indenter of the universal testing machine. One-way analysis of variance and the Post Hoc Tukey test for group comparisons as statistical analysis. Samples with (5.25% NaOCl+Q-mix) displayed the highest bond integrity of BCS (8.00±0.24 MPa). The lowest POBS was demonstrated by the apical section of group 4 (RBP+Q-mix 2 in 1) (4.10±0.94 MPa) specimens. Group 2 (ECYL+Q-mix) and group 3 (LGE+Q-mix) exhibited comparable outcomes of bond scores to group 1 specimens (p > 0.05). MH was significantly lower in group 4 compared to group 1, group 2, and group 3 (p <0.05). The bond strength of BCS and MH of canal dentin is influenced by the type of irrigation.

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