The aim of this study was to investigate the effect of antioxidants on angiogenesis in uterine transplantation. We used 24 female rats equally divided into four groups: Group 1 had the uterus stored in HTK (Histidine-Tryptophan-Ketoglutarate) solution at 4 °C cold storage for 4 h. Group 2 had the uterine tissue stored in HTK solution combined with acetyl L-carnitine (10M) for 4 h at +4 °C. The same procedures with Group 1 and 2 were repeated for 24 h for Groups 3 and 4, respectively. Histological investigation and immunohistochemical analysis were performed. Histological findings showed that storing donor uterus in HTK solution at +4° C for 24 h results in histological alteration in uterus. We also found that immunoreactivity of VEGFR-2 in all layers of rat uterus in Group 2 was lower than that in Group 1, and the expression of the uterus in Group 4 was lower than that in Group 3. We concluded that antioxidant acetyl L-carnitine, which was added to the organ preservation solution HTK, had prevented the formation of free radicals, and thus protected the uterus that was stored in short and long cold storage periods. Impact statement What is already known on this subject? Ischemia-reperfusion is a complex pathophysiological process involve in hypoxia and/or reoxygenation, ionic imbalance-induced oedema and acidosis, oxidative stress, mitochondrial uncoupling, coagulation and endothelium activation. The composition of preservation solutions must be adapted to the severity of ischaemia-reperfusion injuries to reduce cellular damage and inflammation and preserve graft functionality and integrity, thus improving short-term and long-term graft outcome. Clinicians use three types of composition of solution for static cold preservation: intracellular, intermediate and extracellular. HTK will be used frequently, especially with the consideration of lower price and more easy handling aspects. L-carnitine acts as an antioxidant, protects against free radicals and prevents mitochondrial damage. VEGFR-2 plays an important role in angiogenesis, chemotaxis, proliferation and migration of endothelial cells. What this study adds? In this study, we investigate the effect of antioxidants on angiogenesis in uterus transplantation. Our results showed that antioxidant acetyl L-carnitine that added to the organ preservation solution HTK, has prevented the formation of free radicals, thus protect the uterus that was stored in short and long cold storage periods. What the implications are for future studies? Therefore, we will contribute to the literature with the results of this study.

The aim of this study was to investigate the possible effects of melatonin on rat uterine tissue against exposure with bisphenol A (BPA) in the neonatal period. Twenty-four female rats were divided into four groups, (n=6) per group. Group I was used as a control (sesame oil + ethanol), group II was injected daily with (100 mg/kg) BPA by subcutaneously (sc) daily postnatal days (PND 0-10), group III was injected daily with (10 mg/kg) melatonin by sc for 10 days (PND 20-30), and group IV was injected daily with (100 mg/kg) BPA (PND 0-10) and (10 mg/kg) melatonin (PND 20-30). All rats were sacrificed in the same day of metestrus cycle, approximately PND 70. Histological analyses, immunostaining of B cell lymphoma 2 (Bcl-2), and cytochrome c and TUNEL assays were performed. According to our results, neonatal exposure to BPA accelerates onset of puberty, causes degenerative and morphometric changes on rat uterus, and increases apoptotic reaction rates. The immunoreactivity of Bcl-2 was decreased after BPA administration. In addition, immunoreactivity of Bcl-2 showed an increase after melatonin treatment. However, cytochrome c immunoreactivity was decreased after melatonin administration. Our results suggest that melatonin may have positive effects against BPA-induced degenerative changes on rat uterus.

No abstract

Glioblastoma (GB), oldukça agresif ve malign bir beyin tümörüdür. GB, Dünya Sağlık Örgütü (DSÖ) tarafından Evre IV astrositom olarak sınıflandırılmıştır. Tüm primer beyin tümörlerinin %30' unu oluşturan GB (1), her yaşta görülebilmesiyle birlikte genellikle 55-60 yaş aralığındaki erişkinlerde görülmektedir (2). GB'ye neden olan risk faktörü veya faktörleri tam olarak bilinmemekle birlikte GB etkenleri başlıca sigara, elektromanyetik dalgalar, alerji, viral enfeksiyon (3) ve genetik yatkınlık (4) şeklinde ÖZET Amaç: Glioblastoma (GB), oldukça agresif ve malign bir beyin tümörüdür. GB'nin kuratif bir tedavi modalitesi bulunmamaktadır. GB tedavisinin başarısında radyoterapi (RT) uygulamalarının rolü büyüktür. Bu nedenle, kemoterapötik potansiyeli bulunan moleküllerin RT ile kombine uygulanarak hücrelerin radyosensitivitelerinin arttırılmasına yönelik yaklaşımlar önem kazanmaktadır. Epigenetik mekanizmalar hem RT hem de kemoterapi (KT) yanıtında rol almaktadır. Bu çalışmada, bir histon deasetilaz inhibitörü olan sodyum butiratın (SB) RT ile kombine uygulandığında GB hücrelerinde radyosensitiveye olan etkisinin araştırılması amaçlanmıştır. Gereç ve Yöntem: Bu amaçla, LN-405 hücre serilerine farklı konsantrasyonlarda SB (0.,1, 2, 3, 4, 6 ve 8 mM) ve 8 Gy RT ayrı ayrı ve kombine biçimde uygulanmıştır. RT uygulamasında, 3 boyutlu konformal teknik kullanılmıştır. İnkübasyondan 72 saaat sonra hücre canlılığı MTT ile tespit edilmiştir. Bulgular: SB konsantrasyonuna bağlı ancak RT'den bağımsız hücre canlılığını azalttığı tespit edilmiştir. 0.5, 1 ve 2 mM SB ve RT'nin kombine uygulandığı gruplardaki hücre canlılığı yalnızca SB uygulanan gruplara göre sırasıyla %31,4, %36.9 ve %17.6 oranlarında azaldığı saptandı. Bu sonuçlara göre 0.5, 1, 2, ve 8 mM konsantrayonlardaki SB'nin radyosensitiviteyi arttırdığı tespit edilmiştir. Sonuç: Bu çalışma, SB'nin konsantrasyona bağlı olarak RT uygulamalarının tedavici edici etkilerini arttırabileceğini göstermiştir. Ayrıca, tek fraksiyonda akut radyasyon dozu verilen ve hücre serilerine RT uygulama yöntemi açısından 3 boyutlu konformal RT prosedürlerinin uygulanarak SB'nin radyosensitiviteyi arttıdığı ilk çalışmadır.