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In this study, the Bi2O3 nanoparticles have been successfully synthesized through a facile hydrothermal procedure. The structure of the Bi2O3 nanoparticles was analyzed by Fourier transfer infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The synthesized Bi2O3 nanoparticles have unique properties such as high activity, high purity, and high surface area. Hence, we have reported the Bi2O3 nanoparticles as an efficient, cost-effective and mild catalyst for the synthesis of acridinedione derivatives via one-pot four-component reaction. Also, the effect of morphology of Bi2O3 nanostructure was investigated on catalytic performance. Therefore, Bi2O3 nanoparticles were prepared and applied as a heterogeneous catalyst in the synthesis of acridinedione derivatives. The present approach offers several advantages such as excellent yields within short times, green catalyst and ease of recovery.