A structural zone of Dagh Kopet as sediment (Trough) has created in the late Triassic. There are two different views of Dagh Kopet zone: A) Eurasian theory; B) Gondwana theory. The main fault zones are divided into two groups of foundation faults and overthrust. Abtalkh formation is one of the most important Late Cretaceous rock units in the Kopet Dagh sediment Basin and the geographic location of the study area is "03'43˚37 north latitude and "47'35˚55 the east. The calcareous nan-noplanktons are the first links in the food chain and are the main producers in the seas and oceans. The calcareous Nannoplankton is found in fine sediments such as shale and marl and for study and examination; the presence of trace amounts of sample is enough. The calcareous Lannoplankton is used to identify and introduce Nannofossils of Abtalkh in the bottom of the cut, to determine biozones available at the cutting zone and comparing it with the world standard, to determine their age. In the study section, Nannofossil is in glauconitic sandstone, conglomerate, siltstone, marl, shale, limestone, clay, marl and clay marl and Chuck. Their small size makes that a very small amount of samples are sufficient for the study. The most important method for sample preparation in this study is a smear slide method. In laboratory studies and identification of Nannofossil and counting them, the optical microscope and the object lens 50* and 100* are used. To take pictures of samples, 100× objective lens and digital camera were used. In the course of this study, for the lower part of Abtalkh, Aytamir cutting was studied and 12 genera were identified. The calcareous Nannofossils were recognized and have relatively good diversity and abundance. In general, the greater thickness of the study samples had an average preservation. In some cases, the impact of dissolution was so great that the wall or part of the main sections of the species was corroded.