Objective: The aim of this study is to evaluate the effect of different positions of two dental implants with polyether ether ketone (PEEK) telescopic attachment on the Retention of palateless maxillary overdenture.           Methods: Three edentulous maxillary models were fabricated from cold-cure polymethyl methacrylate resin. Each Model has received two implants as follows: Model 1 (one in each canine region), Model 2 (in right canine and left first premolar area), and Model 3 (in right canine and left second premolar area). One Palateless maxillary denture was constructed over each Model. Ready-made PEEK telescope attachments corresponding to implant abutments were embedded in the inner surface of these overdentures. Initial anterior, posterior, and central retention values of overdentures were estimated and compared with the Retention after 540 cycles of insertion and removal using a digital force gauge.       Results: The highest initial anterior retention value was recorded with Model 1, followed by Model 2 and then Model 3 (8.080, 7.460, and 6.980 N), respectively. On the other hand, the highest posterior and central retention values were recorded for Model 3 (11.060 and 10.680 N), and the lowest value was with Model 1 (9.18 and 9.660 N). The highest loss of secondary retention value was recorded with central retention value (24%), then anterior retention value (19%), and the lowest was recorded with posterior retention value (13%).   Conclusions: The implant location and distribution affect the retention value of palateless maxillary overdenture. Continuous insertion and removal of the prosthesis for daily hygiene practice lead to decreases in the retention values.