Three biocontrol agents viz., Trichoderma viride, T. virens and T. harzianum were evaluated to test the antagonism against Sclerotium rolfsii under in vitro conditions. All the three antagonists' viz., T. viride, T. virens and T. harzianum have shown the potential of parasitizing the growth of Sclerotium rolfsii in vitro. The rate of inhibition was fastest in T. harzianum (63.60%) followed by T virens (51.5 %). Least inhibition was recorded in T. viride (50.85% ) after 72 hours of incubation. However, T. viride showed the highest (91.31%) reduction in sclerotia formation followed by T. harzianum (84.92%) and T. virens (84.29%) after 15 days of incubation. The volatile compounds from Trichoderma viride were found most effective in suppressing the mycelial growth (51.11%) and sclerotia production (95.90%) of the target pathogen. The culture filtrate from both T. harzianum and T. viride (15% concentration) was found very effective in inhibiting the radial growth (57.46 and 49.62%) and sclerotia formation (98.20 and 99.83%) of Sclerotium rolfsii. The antagonists such as T. harzianum and T. viride can be used as a bio-control agent against S. rolfsii under field condition.

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Six biocontrol treatments viz., Trichoderma viride, Trichoderma virens, Trichoderma harzianum, T. harzianum + T. viride, T. harzianum + T. virens and T. viride + T. virens were evaluated to test the antagonism against Fusarium verticillioides under in vitro conditions. The maximum growth inhibition (90.6%) was recorded in consortium of T. harzianum + T. viride in dual culture technique . The volatile and non volatile compounds from the consortium of T. harzianum + T. viride also found best and suppressed the mycelial growth of F. verticillioides to the tune of 83.90 and 84.61 %, respectively. Bangladesh J. Bot. 50(2): 423-425, 2021 (June)

An experiment was carried out to study the effect of Meloidogyne incognitaon growth of Pisum sativum (Cv. Kashi Nandini) under glass house conditions by inoculating different inoculums levels i.e. 0 (control), 10, 100, 1000, 10,000 second stage juveniles (J2) of M. incognita along with supernatant. The results revealed that the increase in inoculum level of M. incognita, there was a progressive decrease in growth parameters of the crop as the levels of inocula increased. Significant reduction in plant height, root length and fresh and dry weights of roots and shoots were declined. Maximum galls, egg masses and eggs per egg mass were recorded more pronounced and significant reduction was observed at higher inoculum levels i.e. 1000 and 10,000 J2/pot.

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