No abstract

Triterpenic saponins isolated from seeds of Madhuca indica and fruit pericarp from Sapindus mukorossi exhibited inhibitory effect against two phyto-parasitic nematodes. Azadirachtin and salanin-nimbin-desacetylnimbin (SND) was extracted from seeds and oil of Azadirachta indica A. Juss, respectively. M. indica and S. mukorossi saponins were found to inhibit the movement of pre-adult (J 4 ) stage of Rotylenchulus reniformis with LC 50 of 168.8 and 181.9 mg/mL. Azadirachtin and SND affected the mobility of secondary juvenile stage (J 2 ) of Meloidogyne incognita by 83.3 and 80.1% respectively, at 0.5 mg m/L. M. indica saponin (LC 50 220 mg/mL) exhibited a potentiation effect in the presence of azadirachtin in a 1:3 ratio (LC 50 120.1 mg/mL). A binary mixture (1:1) of azadirachtin and SND was found to show significant nematicidal activity against M. incognita (LC 50 70.9 mg/mL) and R. reniformis (LC 50 91.2 mg/mL).