Bonskr©ki¢ acid causes fatal food poisoninf, whicl~ isassociated with hyl~rlllycaemia, Here we demonstrate thai bonltkrekl,¢ acid, a potent taifibitor of the mltoehondrial ATP~ADP translocase, inhibits( ~)lucose.indtteed electrical activity in the pancreatlt://.ceil through the sti~ulation of ATPsensitive potassium channel (K-ATP.channel) activity. B~' comparison of its effects vcith those of olillomycin, we sulj~est th~,t bonskrekic acid acts by the inhibition of |litlcose metabolism and may induce hyperlllycaemia by impairin~//.cell function, Bongkrekic acid: Metabolism; ATP.sensitiv¢ potassium channel: Pancreatic #.cell

Pada penelitian ini, purwoceng diberikan pada tikus betina bunting umur 1-13 hari dengan tujuan mengetahui bobot badan, ovarium, dan uterus serta mempertahankan titik implantasi yang terbentuk sebelum masa implantasi. Penelitian ini menggunakan 2 kelompok yaitu kelompok tikus bunting kontrol dan tikus bunting yang mendapatkan purwoceng dengan dosis 25 mg/300 g bobot badan. Hasil penelitian menunjukkan bahwa kelompok tikus yang diberi purwoceng cenderung memiliki bobot ovarium dan uterus lebih berat dibandingkan kelompok kontrol. Purwoceng juga menyebabkan jumlah titik implantasi tikus hampir mendekati jumlah korpus luteum yang sudah terbentuk, dan ini menunjukkan bahwa keberhasilan implantasi lebih baik pada kelompok tikus yang diberi purwoceng.

Valproic acid (VPA) plays a role in histone modifications that eventually inhibit the activity of histone deacetylase (HDAC), and will affect the expressions of genes Pdx1, Nkx6.1, and Ngn3 during pancreatic organogenesis. This experiment was designed to study the effect of VPA exposure in pregnant rats on the activity of HDAC that controls the expression of genes regulating the development of beta cells in the pancreas to synthesize and secrete insulin. This study used 30 pregnant Sprague-Dawley rats, divided into 4 groups, as follows: (1) a control group of pregnant rats without VPA administration, (2) pregnant rats administered with 250 mg VPA on day 10 of pregnancy, (3) pregnant rats administered with 250 mg VPA on day 13 of pregnancy, and (4) pregnant rats administered with 250 mg VPA on day 16 of pregnancy. Eighty-four newborn rats born to control rats and rats administered with VPA on days 10, 13, and 16 of pregnancy were used to measure serum glucose, insulin, DNA, RNA, and ratio of RNA/DNA concentrations in the pancreas and to observe the microscopical condition of the pancreas at the ages of 4 to 32 weeks postpartum with 4-week intervals. The results showed that at the age of 32 weeks, the offspring of pregnant rats administered with 250 mg VPA on days 10, 13, and 16 of pregnancy had higher serum glucose concentrations and lower serum insulin concentrations, followed by decreased concentrations of RNA, and the ratio of RNA/DNA in the pancreas. Microscopical observations showed that the pancreas of the rats born to pregnant rats administered with VPA during pregnancy had low immunoreaction to insulin. The exposure of pregnant rats to VPA during pregnancy disturbs organogenesis of the pancreas of the embryos that eventually disturb the insulin production in the beta cells indicated by the decreased insulin secretion during postnatal life.

No abstract

No abstract