The study investigates the effects of pomegranate peel extracts on some virulence factors such as the production of some enzymes produced by Proteus spp isolated from different clinical samples. Among 310 different clinical samples, 48 isolates of proteus were obtained, 14 isolates(29.16%)were obtained from urine, 15 isolates (31.25%) were obtained from stool, 7 isolates (14.58%) from ear swabs, 4 isolates (8.33%) were obtained from wounds, 5 isolates (10.41% (from burns and 3 isolates) 6.25%) of the high cervical. The study showed that from 48 isolates of Proteus spp 25 isolates (52.08%) identified as P.mirabilis, 15 isolates (31.25%)of P. penerri, while 8 isolates of P. vulgaris were obtained with a percentage of (16.66%) and it was found that 18 isolates (72%) of P.mirabilis isolates were positive for the production of beta-lactamase enzyme, 24 isolates (96%) are positive for proteases and 14 isolates (56%) produced lipase, while 13 isolates from P. penerri (86.66%) produced beta-lactamases and proteases and 10 isolates (66.66%) produced lipase, also found that 5 isolates (62.5%) of P. vulgaris produced Beta-lactamase and all the isolates (100%) produced protease while 7 isolates (87.5%) produced lipase. It was found that the hot aqueous extract of pomegranate peels inhibited the production of beta-lactamase enzyme in 4 out of 7 isolates that produced this enzyme, while all tested isolates did not produce lipase by using extracts of pomegranate peel extracts. The isolates lost their ability to produce protease enzyme, except for two isolates that were not affected by extracts of pomegranate peel fruits.

The study included isolation and identification of Proteus mirabilis from different clinical samples, as it was obtained 25 isolates belonging to P.mirabilis (8.06 %) of the total (310) samples taken from the outpatients' and inpatients in Kirkuk General Hospital and Azadi education hospital whom infected with urinary tract , diarrheagenital tract infections , wounds , burns and ear and of different ages and both gender. P. mirabilis diagnosed depending on morphological and biochemical tests as well as by using the Rapid ONE system. The results indicated that all isolates showed swarming on the surface of blood ager and was gram negative also the isolates showed variation in the production of hemolysin that increase the pathgenicity and the results showed resistance of most isolates to antibiotics Amoxillin and Ampicillin and Amoxillin _Clavulanic acid and Azithromycin and Cefixime, while most of the isolates were sensitive to Amikacine Piprecilline and Chloramphenicol and Ciprofloxacin. The results of this study showed sensitivity of P.merabilis to hot aqueous extracts of pomegranate peel in dilution (50.25 , 12.5)mg\ml , also the study showed the effect these Proteus merabilis Pomogranate peel Swarming , Hemolysin. keywords : dilutions on the diameters of (Swarming) and the ability of bacteria to produce hemolysin.