We successfully constructed a reliable SSH library of BTCC and found that combination detection insulin-like growth factor 1 (IGF-1), hTERT, BLCA-4 and HOXA13 genes could help to evaluate BTCC at different stages.

Background/Aims: To investigate pre- and postoperative expression and alterations in the indices of peripheral blood lymphocytes (PBL) CD25 and Ki67 in 32 patients with renal cell carcinoma (RCC) who underwent unilateral radical nephrectomy. We investigated the relationship between the above parameters in PBL and CD8+Ki67+ T cells infiltrated in tumor cells. Patients and Methods: Blood samples were collected preoperatively and on postoperative day 7 (POD7). The PBL CD25 and Ki67 indices were detected by flow cytometry in 32 patients with RCC and in 16 healthy individuals. We conducted double immunohistochemistry for CD8 and Ki67 in tumoral, peritumoral and normal kidney tissues. Results: The PBL CD25 and Ki67 indices on POD7 were higher than their preoperative levels (CD25: 21.37 ± 4.66 vs. 20.30 ± 6.23%; Ki67: 21.92 ± 16.08 vs. 17.05 ± 9.87%), with no significance (p = 0.397 and 0.076, respectively). The pre- and postoperative PBL CD25 and Ki67 indices were significantly higher in patients than in the control group (where they were 14.72 ± 5.40 and 7.50 ± 5.25, respectively, p < 0.01). A negative correlation was observed between the level of the PBL CD25 index on POD7 and the expression of CD8+Ki67+ T cells infiltrated in tumor cells (r = –0.417 and –0.657, p = 0.017 and 0.000, respectively). Conclusions: The PBL CD25 and Ki67 indices could be useful parameters to evaluate performance status in RCC patients. The data we obtained seemed controversial and puzzling and further investigation is required.

Objective:To study the expression of dendritic cells in human renal cell carcinoma and explore the cause, so to reveal the mechanism of escaping immune surveillance in RCC. Methods: The expressions of CD83+DCs, CDla+DCs,VEGF and TGF-I31 in tumoral, peritumoral and normal kidney tissues of RCC in 30 cases were detected by immunohistochemistry using streptavidin/peroxidese(SP) Results: CD83+DCs were mainly located in the peritumoral areas; whereas CDla+DCs, were mainly retained within the cancer nests. The number of CD83+DCs was inversely correlated with the clinical stage (P<0.05); but there were no significant correlations between the number of CD 1 a+DCs, and the clinical stage ( P>0.05 ). The expressions of CD83+DCs and CDla+DCs have significant difference between the tumoral, peritumoral and normal kidney tissues(P<0.001). The expression of VEGF and TGF-131 were significantly lower in samples with highly infiltrating CD83+DCs(P<0.05); Whereas CDIa+DCs were not (P>0.05). Conclusion: DC has the tendency to gathering in tumor, but because of the immunosuppressive cytokins, for example VEGF and TGF-[31, inhibits the maturation of DC, there are less mature TIDCs(CD83+TIDCs) in the tumoral tissues, they are mainly located in the peritumoral areas. This may contribute to the mechanism of escaping immune surveillance in RCC.