Background: Blood group and its relation to bleeding time (BT) and clotting time (CT) is important in conditions like epistaxis, thrombosis, and surgery. Earlier studies depicted O group having prolonged BT and CT. The aim of this study was to assess the relationship of BT and CT with ABO groups.Methods: This cross-sectional study was conducted in the department of physiology, Gauhati medical college. The study included 154 students. Blood grouping was determined with the standard antiserum; BT and CT were estimated by the duke method and slide method, respectively. Blood group and its relation to BT, CT were analyzed by Chi-square analysis.Results: Blood group O (42.2%) was predominant in both genders followed by B (31.8%), A (21%), AB (4.5%). CT was found to be more than 6 minutes in group O (31.57%) followed by group AB (26.31%), A and B (21.05%). The difference was statistically significant (p = 0.02). BT was found to be more than 4 minutes in group O (82.35%) followed by A (11.76%), B (5.88%), and AB (0%). That was statistically significant (p = 0.01). CT was more than 6 minutes in 57.89% in females as compared to 42.10% in males, variation was statistically significant (p = 0.01). BT was more than 4 minutes in 58.82% females as compared to 41.17% in males. The variation was statistically significant (p = 0.03).Conclusions: In present study blood group O was more common followed by B, A, and AB. CT and BT were prolonged in O group. BT and CT were more in females than males.