A BSTRACT The use of salivary biomarkers in diagnosis, treatment, and overall prognosis of coronavirus disease 2019 (COVID-19) has been developed recently. Salivary biomarkers are extremely promising as they are fast to obtain and involve noninvasive collection of specimens. Monitoring patients in real time is necessary in this pandemic. Saliva is another biofluid with major advantages at the molecular level. Methods that detect viral presence in the host secretions measure the current infection by severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2), whereas the detection of human antibodies against SARS-CoV-2 evaluates the past exposure to the virus. There is an urgent need to increase the active research for the detection of SARS-CoV-2 in saliva because diagnostics may provide a reliable and cost-effective method and is suitable for the fast and early detection of COVID-19 infection. Salivary biomarkers have a potential to be a vital guide in determining coronavirus disease. Many people still do not get results of COVID-19 tests due to imbalance between supply and demand at large testing centers. The use of saliva has various advantages compared to collection of nasopharyngeal swabs. New techniques should be developed for detecting salivary biomarkers that help in diagnosis of COVID-19.

Background: The key goal of the study is to find out the effectiveness of anacardic acid and the stannous fluoride as an anti erosive agent. Objectives: To evaluate the erosive effect of Bio vinegar and antierosive effect of Anacardic acid and Stannous Fluoride.    Methodology: The extracted teeth were collected for in vitro study. Three solutions were selected Bio vinegar, Anacardic acid, Fluoride to treat the extracted teeth to study and compare the anti-erosive effect. The sets of extracted teeth of ten each were grouped to be treated with different solutions. The set was immersed in Bio vinegar for 8 hours for erosive action. The second set of ten extracted teeth were immersed in anacardic acid for four days. The same set of teeth were treated with vinegar for 8 hours. The cross sectional view of stained teeth was viewed under Scanning Electron Microscope. The third set of teeth was immersed in stannous fluoride for four days and then treated with Bio vinegar for 8 hours. The cross sectional view of stained teeth was viewed under Scanning Electron Microscope. A comparison of anti-erosive effect of anacardic acid and stannous fluoride were studied under Scanning Electron Microscope. Expected Results: Determining a better effectiveness of Anacardic acid antierosive agent than stannous fluoride. Conclusion: Reduction in the level of erosion by the application of anacardic acid and stannous fluoride on extracted teeth exposed to carbonated drinks and assessment of erosive effect of carbonated drink on tooth.

Dental erosion is defined as an irreversible loss of dental hard tissue due to exposure to chelating agents or non-bacterial acids. The occurrence of this condition was noted and the incidence and prevalence of dental erosion has been increasingly documented.The Ant erosive agents such as Anacardic acid in which the key component is the cashewnut shell liquid is phenolic lipids. It is a mixture of molecules which are saturated and unsaturated. It is also considered to have an anti-microbial effect and has been studied for the treatment of cancer, oxidative damage, inflammation and obesity disorders. Other anti-erosive agent like Fluoride helps in tooth remineralization. Fluorapatite, rather than hydroxyapatite, forms during the process of remineralization when fluoride is found in oral fluids. In apatite crystal lattice formation, fluoride ions replace hydroxy ions. Fluorapatite, even under acidic conditions, is less soluble than hydroxyapatite, which helps to regenerate tooth enamel. Fluoride is therefore a stronger anti-erosive agent. Various Recent advances in anti-erosive agents are Calcium and phosphate, Casein phosphopeptide amorphous calcium phosphate (CPP-ACP), Protease inhibitors, Oils, Chitosan chitosan and Multivalent metal ions Various techniques to evaluate dental erosion are in vitro techniques and in vivo techniques. In vitro techniques are Scanning electron microscope, Surface Profilometry, Polarized Light Microscopyand Non-Contact Confocal Laser Scanning Microscopy (CLSM). And iv vivo techniques are Photographs Clinical review and indices. The most important point of treatment is identifying and removing the erosion factor, above all current materials and methods. Therefore, early identification of the lesions, evaluation and removal of the etiological variables are relevant topics.