The changes of chemical composition and sucrose content in sugar beet beet after seeds stimulation were investigated. The seeds came from the energ'hill technology (Eh) and were subject to laser irradiation. The experiments were conducted in the laboratory and in field conditions in 2008-2010. In the experiment the researchers used the seed material of Tiziana variety (sugar type) in standard version (control) and stimulated: prepared in the energ'hill technology and irradiated. Irradiation with laser light was applied in the following doses: the 5-time multiplied (in the study marked as D5) and 7-time multiplied (in the study D7) of the basic dose 2.5 10-1 J cm-2. The content of nitrogen, phosphorus, potassium, sodium, magnesium and calcium in leaves and roots samples collected in the first weeks of July, August, September and October. Pre-sowing stimulation with use of energ'hill technology and laser light irradiation resulted in an increase in leaf concentration of nitrogen, potassium, sodium and magnesium and the content of these elements positively correlated with sucrose content of mature roots. Pre-preparation (sprouted) clusters energ'hill technology and laser irradiation had a positive effect on sucrose content and its performance.