Background: Since primitive times, man has gone in different ways to search for cures and relief from various diseases by using several plants, plant products and plant-derived products. Recently, there is a scientific curiosity and certain popularity with regard to screening essential oils and extracts from plants used medicinally all over the world. Objective: This study design to evaluate the component of ginger extracts in different solvents by HPLC in addition to evaluate the in vitro effects of it on microorganisms , especially the plant is used in Iraqi folk medicine as anti-inflammatory, antipyretic, anti-diabetic, relieving rheumatic pains and for treating stomach discomfort. Methods: The extraction of soluble compounds from ginger by the soxhlet method was performed by using methanol and n-hexane as solvents and the main composition of ginger extracts were analyzed by HPLC. Agarwell diffusion method tested antimicrobial activities of two crud extracts in different concentrations of plant on seven types of bacteria strains and one fungus. Results: Seven components were identified from the separation of ginger extracts by HPLC. The two extracts had antimicrobial activity, methanol extract was superior than n-hexane extract against the same tested microorganisms. Conclusion: The results of this study revealed that this plant possesses some antimicrobial properties as antibiotics and antifungal, therefore they can be used as a potential source of active ingredients for food, pharmaceutical industry or preservatives.