This study was carried out in the laboratories and fields of Department of Plant Protection, College of Agriculture and aimed at isolating and identifying Trichoderma spp. from different agricultural soils in Basrah and evaluating of the efficacy of some polymeric materials in the formulation of T. longibrachiatum as biofungicides. The results showed that there was no direct effect of Polyvinyl acetate (PVA) on the growth of Rhizoctonia solani and T. longibrachiatum as inhibition percentage was 0%. While Polyurethane (PU) had low effect on growth of T. longibrachiatum as inhibition percent was 4.47%. The results of pot experiment showed that PVA reduced tomato seeds germination to50% compared to 66.6 and 70% in PU and control treatment respectively. The results of bio effect in the control of tomato seedlings disease caused by R. solani showed that T. longibrachiatum +PU (PUT) improve tomato seeds germination and enhanced plant growth as plant height reached 15.7cm compared with 13.5 and 12.9 in PVAT and control treatment. The results also showed that the polymeric material improved longevity of T. longibrachiatum spores for six months, but the spore viability began to decreased gradually during the storage period, reaching 6.03?10? and 2.54?10? cfu.g-1 with PVAT and PUT respectively after six months compared to the number of spores before storage of 5.09 × 10? and 1.77 × 10? cfu.g -1 respectively.