Ferula asafoetida L. has been studied for centuries by many scientists as food products and in traditional medicine, which are relevant for Kazakhstan as well. Recent studies have shown that Ferula asafoetida L. has neuroprotective, memory-enhancing, digestive enzymatic, antioxidant, antispasmodic, hypotensive, hepatoprotective, antimicrobial, antitumor, cytotoxic, and anthelmintic effects. The aim of this study is to determine the component composition by GC-MS method and to study the antimicrobial properties of Ferula asafoetida L. extract obtained by CO2 extraction, which grows in Kazakhstan. Materials and methods. To determine the possibility of using Ferula asafoetida L., we carried out the component composition of the extract obtained by CO2 extraction in subcritical conditions of the underground part of Ferula asafoetida L. by a certain GC-MS method and also investigated the antimicrobial effect of this extract. Results. The plant raw materials were collected in accordance with GACP requirements. Conducted subcritical CO2 extraction of plant raw materials showed a 2.5% extraction yield. The study of the component composition by GC-MS revealed 46.3% of sulfate compounds. The determination of antimicrobial activities showed high efficacy against gram-positive (Staphylococcus aureus subsp. Aureus, Bacillus subtilis subsp. Spizizenii), gram-negative bacteria (Escherichia coli, Klebsiella pneumoniae subsp. pneumoniae, Salmonella enterica subsp. enterica) and fungi (Candida albicans, Aspergillus niger). Conclusions. The possibility of using the obtained CO2 extract of Ferula asafoetida L. in the field of pharmaceutical products as a substance and a drug that has a huge antimicrobial effect