Myoma uteri related to the interaction between genes, hormones, growth factors, cytokines, and environment. Myomas are not found before menarche, whereas after menopause only 10% of myomas grow. The development of uterine myoma depends on estrogen. CYP1A1 is one of three members of the CYP1 family that is involved in the metabolism of a large number of xenobiotics. The purpose of this study was to analyze the relationship between the CYP1A1 gene polymorphism (Ile462Val) and the incidence of uterine myoma in ethnic Malays in South Sumatera. This research is an observational study with a case control design. The blood samples taken from 35 cases and 35 controls. The data was analyzed using the Chi-square test. The results indicate that there is a significant relationship between the use of contraceptives on the incidence of uterine myoma. There is a significant relationship between the CYP1A1 gene polymorphism (Ile462Val) and the CYP1A1 allele (Ile462Val) with uterine myoma. It is concluded that there is a polymorphism relationship between the genotype and allele of the CYP1A1 gene (Ile462Val) and the incidence of uterine myoma in ethnic Malays in South Sumatera.