The sterile insect technique (SIT) is based on the inundatory field release of a target pest following their reproductive sterilization via exposure to radiation. Until recently, gamma irradiation from isotopic sources has been the most widely used in SIT programs. As isotopic sources are becoming increasingly expensive, especially for small programs, and regulations surrounding their procurement and shipment increasingly strict, irradiation capacity is one of the limiting factors in smaller or newly developing SIT projects. For this reason, the possibility of using X-ray irradiators has been evaluated in the recent decade. The availability of “off-the-shelf” blood X-ray irradiators that meet the technical requirements for insect irradiation can provide irradiation capacity for those SIT projects in which the acquisition of gamma ray irradiators is not feasible. Following the recent technical characterization of a Raycell MK2 X-ray blood irradiator, it was found in this study, that MK2 instruments were suitable for the sterilization of fruit flies, tsetse flies and mosquitoes, inducing comparable, even slightly higher, sterility levels compared to those achieved by gamma ray irradiation. This, together with its estimated processing efficiency, shows that MK2 irradiators are suitable for small- to mid-sized SIT programs.
Tsetse flies are cyclical vectors of trypanosomes, the causative agents of sleeping sickness or Human African Trypanosomosis and nagana or African Animal Trypanosomosis in Sub-Saharan Africa. The Insectarium de Bobo-Dioulasso (IBD) was created and equipped in the frame of Pan African Tsetse and Trypanosomosis Eradication Campaign (PATTEC) with the main goal to provide sterile males for the different eradication programs in West Africa which is already the case with the ongoing eradication program in Senegal. The aim of this study was to identify the best feeding regime in mass-rearing colonies of Glossina palpalis gambiensis to optimize the yield of sterile males. We investigated the mortality and fecundity for various feeding regimes and day alternation (3×: Monday-Wednesday-Friday, 4×: Monday-Wednesday-Friday-Saturday, 4×: Monday-Wednesday-Thursday-Friday and 6×: all days except Sunday) on adult tsetse flies in routine rearing over 60 days after emergence. The day alternation in the 4 blood meals per week (feeding regimes 2 and 3) had no effect on tsetse fly mortality and fecundity. The best feeding regime was the regime of 4 blood meals per week which resulted in higher significant fecundity (PPIF = 2.5; P = 0.003) combined with lower mortality of females (P = 0.0003) than the 3 blood meals per week (PPIF = 2.0) and in similar fecundity (PPIF = 2.6; P = 0.70) and mortality (P = 0.51) than the 6 blood meals per week. This feeding regime was extended to the whole colonies, resulting in an improved yield of sterile males for the ongoing eradication program in Senegal and would be more cost-effective for the implementation of the next-coming sterile insect technique (SIT) programs in West Africa.
Beekeeping is a potential source of income for rural populations. Once viewed as a minor agrosylvopastoral activity, beekeeping is now considered a promising activity. However, data to guide interventions are insufficient. The objective of this study was to carry out a technical and socioeconomic characterization of beekeeping in the Center-West Region of Burkina Faso. A cross-sectional survey using a pretested questionnaire enabled to collect information on the socioeconomic and demographic characteristics of beekeepers (n = 113), and the characteristics of apiaries and beekeeping practices. The results showed that the majority of beekeepers were male (64.3%), educated (71.4%), with an average age of 42.8 ± 10.4 years, belonging mainly to the Mossi ethnic group (76.9%). Practiced as a secondary activity, the main attraction of beekeeping was its profitability for 69.0% of those interviewed. With a dominance of modern Kenyan type hives, the typology identified three production systems: the modern system, the traditional system and the mixed system. Average production was 6.9 ± 2.9 and 11.5 ± 2.8 liters per year for the traditional and modern hives, respectively. The profitability of the apiaries reached 10,504 and 24,620 CFA francs annual net margin for the traditional and modern hives, respectively. The main constraints revealed by the study were the lack of modern equipment and financial resources, insufficient technical supervision, and pests/predators.
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