In this study, it was aimed to investigate the antioxidant and antibacterial capacity of the rosehip plant grown in Samsun and its surroundings. In the study, rosehip fruits were collected from 10 different regions in and around Samsun. In order to investigate the antioxidant capacity of the samples, ascorbic acid amount in grams (g) ascorbic acid, total tannin amount in gram tannic acid, total flavonoid amount in mg quercetin, total phenolic substance amount in gram gallic acid equivalent (GAE), total protein amount in g bovine serum albumin (BSA) /100 g of rosehips; total antioxidant level (TAS) mmol trolox equivalent/L, total oxidant amount was calculated in mmol H2O2 equivalent/L. Disk diffusion method was used to measure the antibacterial capacity of rosehip samples, and the inhibition diameters of the samples were determined in millimeters (mm).In line with the findings obtained in our study, the amount of ascorbic acid of the rosehip samples is 0.781-1.120 g ascorbic acid/100 g of rosehip, the amount of tannin is 1.42-4.65 g of tannic acid/100 g of rosehip, the amount of flavonoids is 29.5-36.3 mg of quercetin/100 g of rosehip, the amount of phenolic substance is 4.700 -8.347 g GAE/100 g rosehip, total protein amount 0.54-0.89 g BSA/100 g rosehip, total antioxidant level 2.59-2.62 mmol trolox equivalent/L, total oxidant level 6.13-7.41 mmol/ H2O2 equivalent/L, test Antimicrobial activity against the bacteria was not determined.In this research, it was determined that the rosehip fruit grown in Samsun and its surroundings has a high antioxidant capacity due to the amount of ascorbic acid it contains, and also shows antibacterial activity. As a result of the study, it was concluded that the composition of rosehip fruit and adding it to the feeds of livestock such as cattle, poultry and pigs will have positive effects.

Multidrug-resistant tuberculosis (MDR-TB) is defined as resistance to at least isoniazid (INH) and rifampicin (RIF), and it complicates the implementation of tuberculosis control programmes. The rapid detection of MDR-TB is crucial to reduce the transmission of disease. The nitrate reductase assay (NRA) is one of the colorimetric susceptibility test methods for rapid detection of MDR-TB and based on the ability of reduction of nitrate to nitrite by Mycobacterium tuberculosis. The aim of this study was to evaluate the performance of the NRA for the rapid detection of MDR-TB. A total of 237 M.tuberculosis complex (MTC) isolates that were identified by the same method (BD MGIT(TM) TBc Identification Test, USA) from nine different medical centers in Turkey were included in the study. The susceptibility results of the isolates against INH and RIF obtained by reference test (Bactec MGIT(TM) 960, BD, USA) were then compared with NRA. In order to ensure consistency between centers, Löwenstein-Jensen (LJ) medium with antibiotics and without antibiotics (growth control) and Griess reagent solution were prepared in a single center (Ondokuz Mayıs University School of Medicine, Medical Microbiology Department) and sent to all participant centers with the standardized test procedure. After the inoculation of bacteria into the test tubes, the tubes were incubated at 37°C, and after seven days of incubation, 500 μl Griess reagent was added to the LJ medium without antibiotics. If a color change was observed, an equal volume of Griess reagent was added to test LJ media with antibiotics. When a color change was observed in LJ media with antibiotics, it was considered that the isolate was resistant to tested antibiotics. Among 237 MTC isolates, 16 were resistant only to INH and nine were resistant only to RIF; 93 isolates (39.2%) were resistant (MDR) and 119 isolates (50.2%) were susceptible to both of the drugs determined with the reference susceptibility test. In the study, five INH-resistant isolates determined with reference method were found susceptible with NRT and eight INH-susceptible isolates determined with reference method were found resistant with NRT. In contrast, one RIF-resistant isolate determined with reference method was found susceptible with NRT and three RIF-susceptible determined isolates were found resistant with NRT. Accordingly, the concordance rate between the reference method and NRA were estimated as 94.5% for INH and 98.3% for RIF. The sensitivity, specificity, positive and negative predictive values of NRA were detected as 95.4%, 93.7%, 92.8% and 96% for INH, and 99%, 97.8%, 97.1% and 99.2% for RIF, respectively. The results of the 111 isolates were obtained on the seventh day, while the rest of the results were obtained between 10-14 days. In conclusion, the data of this multicenter study showed that NRA is a reliable, relatively inexpensive and practical method to perform for the rapid detection of MDR-TB.