BACKGROUND Phosphate‐solubilizing bacteria (PSB) can be an environment‐friendly strategy to improve crop production in low‐phosphorus (P) or P‐deficient soils. The effect of indigenous mixed inocula of PSB on Agave angustifolia Haw. growth was assessed. The four treatments evaluated were T1 (Pseudomonas luteola + Enterobacter sp.), T2 (Pseudomonas luteola + Bacillus sp.), T3 (Pseudomonas luteola + Acinetobacter sp.), and T4 (control); each was replicated 25 times using a completely randomized design during 12 months under rain‐fed conditions. Additionally, P solubilization in vitro of the mixed inocula with three different sources of inorganic P was tested. RESULTS The mixed inocula were able to solubilize more P from tricalcium phosphate Ca3(PO4)2 than from aluminum phosphate (AlPO4) and iron phosphate (FePO4). Relative to the control, T2 increased plant height by 22.9%, leaf dry weight by 391.4%, plant stem diameter by 49.6%, and root dry weight by 193.9%. The stem solid soluble content increased 50.0% with T1. Plant‐available soil P increased 94.6% with T3 and 77.3% with T1. Soil alkaline phosphatase activity increased 85.9% with T1. CONCLUSION T2 was the mixed inoculum that most improved Agave angustifolia plant growth. The indigenous mixed inocula of PSB evaluated appears to be a practical and efficient option for promoting field growth of Agave angustifolia plants. However, further research is necessary to achieve a deeper understanding of the relationships between different PSB species and their effects on agave, which may reveal some of the mechanisms of the synergistic interactions that are involved in the promotion of plant growth. © 2019 Society of Chemical Industry