Gölcük Lake (Bozdağ-İzmir) is polluted by the touristic activities and agricultural and domestic sources. Gölcük Lake also adversely impacts human health as the lake water is used for irrigation in agriculture, and the fish caught from the lake is used for nutrition by the community around it. For this purpose, "OECD 201 Algal Growth Inhibition Test", the standard test protocol was evaluated with cultures of green algae Desmodesmus dimorphus as the representative of the first trophic level. This test was used to assess the toxicity of sediment samples collected from five sampling sites in Gölcük Lake. Extracts of sediment samples were assayed in four different concentrations (20, 40, 100, and 200 mg/ml). The effects on the growth of D. dimorphus were examined by scoring cell numbers under the light microscope with a Neubauer haemocytometer counting chamber. According to the results, all extracts, except those from Station 3, inhibited growth of D. dimorphus in increasing concentrations of sediment samples, and inhibition was observed in the following order of stations: 2>5>4>1. Additionally, an increase in the number of algae (hormesis) was observed in the Station 3, depending on time and concentrations. Keywords: Algal Growth Inhibition Test, Pollution, Freshwater, Gölcük LakeÖz: Gölcük Gölü (Bozdağ-İzmir) turizm aktiviteleri, evsel ve tarımsal kaynaklar nedeniyle kirlenmektedir. Gölcük Gölü'nün suyu tarımda sulama amaçlı kullanılmakta ve gölden beslenme amacıyla balık avlandığı için göl suyu, civarındaki halkın sağlığını da olumsuz yönde etkilemektedir. Bu amaçla, "OECD 201 Algal Büyüme İnhibisyon Testi" kısa zamanlı phytotoxicity test yöntemi için standart bir test protokolüdür ve yeşil alglerden olan Desmodesmus dimorphus kültürü birinci trofik seviye temsilcisi olarak değerlendirilmektedir. Bu test, gölden daha önceden belirlenmiş 5 örnekleme yerinden toplanan sediment örneklerinin toksisitesini değerlendirmek için kullanıldı. Sediment örneklerinin ekstraktları dört farklı konsantrasyonda (20, 40, 100 ve 200 mg/ml) test edildiler. Desmodesmus dimorphus'un büyümesi üzerindeki etkiler, sayma kamarası Neubauer hemositometre ile ışık mikroskopu altında hücre sayımı yapılarak değerlendirilmiştir. Sonuçlara göre, 3 numaralı istasyon dışında tüm sediment ekstraktları artan konsantrasyona bağlı olarak Desmodesmus dimorphus büyümesini engellemiştir ve bu istasyonlar sırasıyla 2>5>4>1 nolu istasyonlardır. Ayrıca 3 nolu istasyonda zamana ve konsantrasyona bağlı olarak alg sayısında artış (hormesis) gözlenmiştir.

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The sea has involved a large variety of environmental contaminants and plays a crucial role in aquatic ecosystems. The Izmir Bay, which has been rapidly polluted since the 1960s, was one of the intensely polluted areas in the Mediterranean. Organic materials, hydrocarbons, metals, and pathogenic organisms that are accumulated in the region, caused high pollution and threaten health and aesthetics. Because of this, the aim of this paper was focus on to investigations of the genetic damages in mussels in Izmir Bay. Investigations of mutagenic/carcinogenic potential have an advantage in genotoxicity studies because biomarker for pollution exposure in mussel is the early detection of possible long-term effects such as cancer. Therefore, genotoxicity was the focus of the biomarker investigations in mussel during the investigations. Thus, micronucleus tests were afforded to determine genetic damage in the haemolymph, liver, and gills of Mytilus galloprovincialis living in Izmir Bay (Western Coast of Turkey). In the present study, results showed that the frequency of MN was found at a high level in station 4 and station 5 where wastes from dockyard existed contributed to the high level of pollution. The recommendation is to standardize procedures for assessment of the toxic impact of pollutants at the cellular level in aquatic species by using micronucleus assays for biomonitoring of environmental pollution.

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