The primary reason for an endodontic treatment failure is the persistence or regrowth of different species of bacteria within the root canal system. The root development and closure of its apex in a permanent tooth occurs till 3 years after the eruption. Treatment of traumatic injury of teeth during the development period of root resulting in open apex poses an endodontic challenge. Various materials are used for induction of apical closure to produce favourable conditions and to conne the root canal lling within the canal space. This paper describes clinical case of retreatment on premolar with improperly treated root canal with open apex. Multiple visit endodontic retreatment of premolar was performed. Disinfection was performed using Intracanal diode laser irrigation activation followed by placing triple antibiotic paste (TAP) as intra-canal dressing. In the second appointment apical plug of Biodentine against a platelet-rich brin (matrix) was condensed. The present case showed successful periapical healing and resolution of symptoms with the use of calcium silicate based cement with platelet-rich brin as an apical barrier matrix. Procedure is followed carefully using recommended protocols.