A new simple, accurate and precise HPLC method have been developed and validated for estimation of Zolmitriptan in its pharmaceutical dosage form. In RP-HPLC method, a C18 column and methanol: water in the ratio of 75:25 (v/v %), pH adjusted to 3 using 10% orthophosphoric acid were used at a flow rate of 1.0 mL/min and detected at 222 nm. The retention time for zolmitriptan was found to be 3.6 min. The developed method was validated for linearity, precision, accuracy, specificity, LOD and LOQ as per ICH guidelines. Linearity was observed in the range of 10-50 μg/mL for zolmitriptan and correlation coefficient was found to be 0.9979. LOD and LOQ for Zolmitriptan were found to be 2.84 μg/mL and 8.62 μg/mL respectively. The % recovery was found to be 99.87%-101.57%. The method was applied for estimation of Zolmitriptan in its pharmaceutical dosage form. The assay result was found to be 95.98 ± 1.82 of percentage label claim of Zolmitriptan. aliquot of 0.1, 0.2, 0.3, 0.4 and 0.5 mL were transferred in series of 10 mL volumetric flask and diluted up to mark with mobile phase to get the Figure 1: HPLC Instrument.FT-IR spectrophotometer: BRUKER, Alpha, Electronic analytical balance (Shimadzu, AUX-220) were used (Figure 1). Reagents and materialsPharmaceutical grade of Zolmitriptan was supplied as a gift sample. Methanol was used of LR grade and purchased from s.d. Fine Chem Limited, Mumbai, India. Zolmitriptan nasal spray (ZOLMIST) was procured from the local market. Preparation of standard solutionsPreparation of stock solution of ZMT: 25 mg of Zolmitriptan was weighed and transferred in 25 mL volumetric flask and volume made up to mark with methanol (1000 μg/mL). Preparation of working standard solution:From stock solution of ZMT,