Background: RtcB is a new RNA ligase implicated in tRNA splicing and RNA repair. Results: RtcB has 2Ј,3Ј-cyclic phosphodiesterase and 3Ј-phosphate/5Ј-hydroxyl ligase activities. Conclusion: RtcB seals broken RNAs via a two-step reaction pathway. Significance: The structure, active site, and chemical mechanism of RtcB are unique.
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