Protoplasts of Streptomyces flavopersicus with the highest regeneration frequency were isolated from late log phase mycelium grown in a two-stage culture system with 2% glycine in the medium. Of the 11 regeneration media tested, R9 was selected as the most efficient with 29 degrees C as the best temperature.

The research presents the advantages of using information and communication technologies in education. New applications are described which help in easier learning of the educational material. The opportunities that ICT provide for presenting information in an interesting way to motivate students are presented. Information and communication technologies (ICT) are an essential part of our daily lives. They have entered education at a very fast rate. School education has also changed in a short time. Teachers aim to use new methods and information and communication technologies to arouse students' interest in the educational process.

The research presents the creative world in the stories of Elin Pelin. The period of his development as a writer is tracked. The characters are presented with their positive and negative features. Described are the dialogue, which is a distinctive feature of the author’s stories, the role of the landscapes without which the development of the action is unthinkable, and the atmosphere in the Bulgarian village.