Introduction: Mouth wash are generally utilized as subordinates to oral cleanliness and in the conveyance of dynamic specialists to the teeth and gums. These flushes can impact plaque development and adjust the course of gingival irritation. Azadirachta indica (Neem) was utilized to treat different skin illnesses, as a disinfectant substance and as a natural mouthwash. Stevia rebaudiana can inhibit the growth of microorganisms that are responsible for dental caries. Aim: This study aimed to assess the antimicrobial activity and cytotoxicity from Neem and Stevia based Mouthwash. Materials and Methods: Plant extract was prepared and an antimicrobial and cytotoxic effect was done by considering various parameters. The antimicrobial activity of nanoparticles prepared using plant extract was investigated and the results of the test were described as the standard deviation and analyzed. For the cytotoxic activity, an ELISA plate was used, wherein the mortality rate of the nauplii was estimated with the plant extract mediated nanoparticles at different concentrations. Data’s were statistically analyzed by Spearman correlation through SPSS version 23. Results: Anti-microbial activity showed positive correlation with increase in concentration (r=1). The cytotoxic activity showed negative correlation with the number of live nauplii decreased in the second day when compared to the first day suggesting that the extract has potent cytotoxic activity (r=-1). Conclusion: Neem and Stevia extract helped us to detect the antimicrobial activity and cytotoxic effect on the various species in different concentration levels. The study needs to be evaluated further for isolating the possible compounds to test the effectiveness of antimicrobial activity in the oral cavity of the human body to prevent various diseases.

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Cadaveric donation comprises body donation, organ donation that is, taking out organs (heart, lungs, kidneys, liver, pancreas) from brain dead people, as well as tissue donation, meaning taking tissues (skin, corneas, tendons, bone) from brain dead as well as heart dead people.In a broader sense, however, pathologists are involved in cadaveric tissue donation as well as taking tissues from cadavers for diagnostic procedures within the framework of the autopsy (fluids, organs, tissue samples), and to gather material for research and training students and pathology residents (tissues, organs). For cadaveric tissue donation, different ethical and practical issues must be considered, which we will try to review in this paper from the perspective of the pathologist. It is possible to donate your body to medical science after your death. To make the arrangements, contact the anatomical institute of one of the universities in India , as this is a separate process to organ or tissue donor registration. The aim of the study is to evaluate the knowledge and awareness of organ donation. Analytic, longitudinal and quantitative study, conducted in 5 Medical/Dental College in Natal/RN, between November 2019 and January 2020. Survey has been conducted among dental and medical students 105 responses have been received from 5 medical and dental colleges. Regarding the donation structure, there were deficiencies of physical resources (temperature control), materials (mobile radiology) , human resources (nurse technicians) and lack of adequate records and care protocols. In the process of donation, the biggest problems were related to the evaluation stages, brain death diagnosis, maintenance and documentation, with greater proportion of care for the non-donor. 85.4% of the respondents were aware of Organ donation whereas 14.6% of the study population were not aware about organ donation. The primary outcome was intention to donate. Taking a donor card after the interview was a secondary behavioral outcome.