Cladistic analyses by maximum parsimony and Bayesian inference methods of chloroplast and nuclear sequence data indicate a new position for Limosella (Scrophulariaceae). Following this result, a new circumscription of the tribe Limoselleae is presented where the tribe Manuleeae is included in Limoselleae. Further, the study discloses that the genus Sutera is paraphyletic in its present circumscription, but that the two sections of Sutera , Sutera and Chaenostoma , are monophyletic. To accommodate these findings the genus Chaenostoma is re-established. Furthermore, the genus Jamesbrittenia recently expanded by Hilliard is shown to be a highly supported monophyletic group in its current circumscription.