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Scand J Clin Lab Invest Downloaded from informahealthcare.com by Chulalongkorn University on 12/26/14For personal use only.

Information on purines in the urine of human beings is rather scanty, as the analytical meth,ods were comparatively complicated. In an earlier publication (Brandt Petersen, J$rni & J$rgensen 1965) a method was described for the specific enzymatic measurement of hypoxanthine (6-oxypurine) and xanthine (2.6-oxypurine) in urine.The results of the measurements of hypoxanthine (Hx), xanthine (X) and uric acid (U) in the urine of 11 men aged 25-57 years are presented here. The concentration of these oxypurines was followed in the urine for 6 7 consecutive days. I n addition the oxypurine content was measured in a single decomposed blood sample from each of the examined experimental subjects. The measurements were enzymato-spectrophotometric.MATERIAL AND METHODS Two grozps were studied; the first consisted of 5 healthy men, who carried on with their normal daily work on a normal diet. Only one aliquot of an early morning urine specimen was analysed owing to the practical difficulties of collecting a 24-hour urine.In the second group consisting of 6 men, who were subjected to herniorrhaphy in epidural anal-gesia on the first day of investigation, the 24-hour output of oxypurines in the urine was determined for 6 7 consecutive days. Apart from the cause of operation, these patients were completely healthy ; it is especially noteworthy that the case histories contained no indication of metabolic disorders or blood diseases. They were all admitted the day prior to operation. Apart from the omission of breakfast and lunch on the day of operation these patients were given a normal diet. The herniorrhaphy was carried out on the morning of the first day of observation and was as a rule of 1-1s hours duration. The anesthesia consisted of 2 per cent lidocain-noradrenalin, 15-20 ml, injected into the epidural space in the lumbar spine. The latter half of the observation period constituted the control against the first 2-3 postoperative days.The analyses were carried out on an aliquot of the 24-hour urine.In addition, a single blood sample was taken from each of the subjects after the expiration of the investigation period.Uric acid was measured by uricase according to Prztorius & Poulsen (1953).Hypoxanthine and xanthine in the urine were measured according to Brandt Petersen et al. (1965).Hyfioxanthine and xanthine in the blood: 4-5 ml of blood were taken, using a sterile technique, into a test tube containing a few drops of heparin solution (5.000 units per ml). The sample was then placed in a thermostat at 37" for 48-72 hours. This results in a complete destruction of the blood purine nucleotides with the formation of a cor-460 Scand J Clin Lab Invest Downloaded from informahealthcare.com by Chulalongkorn University on 01/03/15For personal use only.