The purpose of the study was to investigate the impact of stress and burnout on the quality of life of pastors in Assemblies of God Church in the Ashanti Region, Ghana. A descriptive survey design was adopted for the study. A sample of 254 out of a population of 744 was used for the study using stratified and simple random sampling procedures. Data were collected using a questionnaire and analysed using means and standard deviations, linear multiple regression, independent samples t-test, and one-way Analysis of Variance. Results showed that the pastors often experienced stress and frustrations due to a lack of resources such as money and infrastructure, different roles competing for their attention (ministry and family), delays in reaching their ministry goals, and carrying out a lot of activities the same time. The study also found that the pastors felt emotionally drained by their work and felt burned out. The study revealed that the relationship between stress level and quality of life was statistically significant. Similarly, the relationship between burnout level and quality of life was statistically significant. It was recommended that the leadership of Assemblies of God Church, Ghana should organise intermittent workshops for their pastors in all their branches on how to cope with the emotional drains of pastoring.