This paper presents a case of disseminated sporotrichosis in a 13-year-old female, originating from a rural area in Minas Gerais state, Brazil. The patient was hospitalized in Santa Casa hospital of Belo Horizonte, with hyporexia, prostration, fever and disseminated ulcerative lesions, besides anemia, leucopenia and sepsis of probable cutaneous focus. The patient was admitted without proven immunosuppression. She was diagnosed with cutaneous-disseminated sporotrichosis. The drug therapy chosen was itraconazole during 12 months, leading to important clinical improvement and healing of cutaneous lesions.

Background: Diseases associated to Candida spp. are recurrent and can be difficult to treat, mainly due to the new strains resistant to the limited number of available antifungals. Objective: Evaluate the in vitro and in vivo activity of Brazilian green propolis (GrProp) and red propolis (RdProp) ethanolic extracts against standard strains of Candida albicans, C. tropicalis, C. krusei, C. glabrata, C. parapsilosis and 10 clinical isolates of C. albicans. Methods: Antifungal activity in vitro was tested using the M-27-A3/CLSI protocol. The in vivo antifungal activity was evaluated using Tenebrio molitor model. And, the effect of extracts on adhesion of C. albicans in human buccal epithelial cells (BECs) was also studied. Results: GrProp and RdProp exhibited antifungal activity against at least one of the Candida strains tested. The adhesion inhibition of C. albicans in BECs was of 45% (GrProp28), 60% (GrProp50) and of 82% (RdProp), in comparison to amphotericin B (82%). All propolis extracts showed synergistic activity with fluconazole and amphotericin B. GrProp50 (10 mg/kg) showed the better protection of T. molitor, blocking the progression of C. albicans infection, increasing survival and delayed the larvae death. Conclusion: Brazilian GrProp and RdProp extracts inhibit the in vitro C. albicans growth and protect T. molitor against infection by this yeast. The physiochemical parameters found for the analyzed samples were in accordance to the standards established by the Brazilian Legislation for propolis and derivatives. GrProp and RdProp have potential to be used against Candida spp. infections, mainly in association with fluconazole or amphotericin B.

O consumo de bebidas prontas tem apresentado um significativo aumento ultimamente. O objetivo deste estudo foi avaliar qualidade, estabilidade e aceitabilidade de néctar misto nos seus aspectos físico-químicos, sensoriais e microbiológicos. O néctar misto foi elaborado de três polpas congeladas: açaí, acerola e morango, adquiridas em João Pessoa-PB. As análises físico-químicas realizadas a cada 30 dias por 90 dias foram: pH; acidez total titulável (ATT); Sólidos Solúveis e Vitamina C; análises microbiológicas foram: coliformes totais e Salmonella sp. As análises sensoriais de aceitabilidade e intenção de compra foram realizadas com 40 provadores não treinados em duas amostras, uma a 24±2ºC e outra em 15ºC. Os resultados do néctar misto foram: 19,8ºBrix, pH 2,83, acidez total titulável 0,12% e vitamina C 139,75mg/100mL mantendo qualidade sanitária no período de armazenamento. O néctar misto obteve uma boa aceitabilidade nos atributos aparência, aroma e sabor, e médias 7,63; 7,90; 8,08, respectivamente, em temperatura ambiente; em temperatura de refrigeração médias 7,48; 7,60; 8,10, respectivamente. A intenção de compra do néctar misto não teve diferença significativa em temperatura ambiente (4,50) com a refrigerada (4,58). O néctar misto permaneceu estável durante o armazenamento, uma boa fonte de vitamina C com uma boa aceitabilidade e intenção de compra.