Haemosporidians are vector-transmitted intracellular parasites that happen in numerous bird species worldwide and may possibly have important effects for wild bird populations. Studies of haemosporidians most dedicated on Europe and North America, and only newly some study in the Neotropics has been done, where the occurrence and influences of the disease have been less considered and are not understood well. In this study we designed a study in the nestling birds of prey in Mongolia. We sampled blood from 72 raptors at 2 different species and evaluated avian haemosporidian infection by two nested PCR protocol and one Real time PCR protocol. Sequencing a portion of the cytochrome b (cyt b) gene of the parasite. From the sampled birds, 10 % were infected by Plasmodium. Inclusive, our findings advocate a high haemosporidian species richness in the bird community of Mongolia. In view of the frequency of local habitat loss that in this area is living, recognize how avian haemosporidians affect bird populations it is very important; in addition, more exhaustive sampling is required to fully understand the range of avian haemosporidian infection in this area.