Seeds of Brassica napus L. var AC Excel were sorted into four subsamples on the basis of the amount of chlorophyll present. Vigor of the four subsamples was studied and compared using the germination test, accelerated aging test, controlled deterioration test and electrical conductivity test. Laboratory vigor ratings were correlated with field data. Results show that seed chlorophyll had no significant effect on germination. However, seedling growth and performance were negatively affected by high chlorophyll. High chlorophyll subsamples were more sensitive to increased moisture and temperature than low chlorophyll subsamples. Additionally, seed conductivity study indicates that seed chlorophyll content is directly proportional to seed coat porosity. The usefulness of the four vigor test methods in assessing seed quality among chlorophyll seed lots of Brassica napus is also discussed.