Squid Todarodes pacificus suwari gels, set at various temperatures and times, and acid-induced kamaboko gel, which was prepared by soaking suwari gel in 5% acetic acid for 20 h, were studied to evaluate the mechanical properties that are affected by setting conditions. Unset squid meat paste did not form a gel when soaked in acetic acid. The breaking strength of both suwari gel and acid-induced kamaboko gel showed a tendency to increase with setting temperature and time. SDS-PAGE analysis of suwari gel and acid-induced kamaboko gel, which were set at various temperatures and times, showed that myosin heavy chain (MHC) was observed at 30°C only for the first hour. The intensity of the MHC band at 30°C gradually decreased with setting time, while the intensity of the polymer band gradually increased with setting time. These results suggest that the protein-protein bonds in suwari gel affect the final texture of acid-induced kamaboko gel. Based on the analysis of the mechanical properties, and in consideration of the fact that the purpose of this experiment was to reduce energy usage, the best setting condition was determined to be 40°C for 3 h.

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A sterilized mixed herbal drink formula and process was developed for the mixed herbal extracts from Imperata cylindrical (L.) P. Beauv [IC], Murdannia loriformia (Hassk.) Rolla Rao et Kammathy [ML], Hedyotis corymbosa Lamk. [HC] and Orthosiphon aristatus Miq [OA].Increasing extraction temperature and time decreased the antioxidant activity and increased the total potassium and phenolic compound contents of the herb infusions. Using response surface methodology (RSM), the appropriate extraction condition was 74.6°C for 41 minutes. The most acceptable formula contains a % weight ratio (w/w) of 14:35:27:17of IC: ML: HC: OA infusions to 7% (w/w) sucrose. Decreasing the sterilization temperature and increasing the F 0 -value reduced the antioxidant activity and total phenolic compounds. The appropriate sterilization temperature and F 0 were 135°C and 3 minutes.