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Summary To identify novel antioxidant peptides from egg‐white protein and investigate antioxidant mechanism, the hydrolysate of egg‐white protein was purified by ultrafiltration and a Sephadex G‐15 column. The peptides VYLPR, EVYLPR, VEVYLPR and VVEVYLPR were identified from the fraction with the highest antioxidant activity. The Results showed that the peptide VYLPR exhibited the strongest protective effect on HEK‐293 cells. The viability of cells recovered to 97.45 ± 1.98% with pre‐treatment of 20 μm VYLPR. We further investigated antioxidant mechanism. The results showed VYLPR could inhibit lipid peroxidation process, maintain cell membrane integrity, inhibit intracellular LDH activity, reduce MDA content, and improve the activity of antioxidant enzyme T‐SOD and GSH‐Px. This work could help researchers in understanding antioxidant mechanism of peptides and also contribute to developing functional egg‐white product.

The effect of there fining process on the physicochemical properties, fatty acid composition, bioactive components, and antioxidant properties of evening primrose oil (EPO) was investigated. The results showed that the peroxide value, acid value, and p-anisidine value changed significantly throughout the refining process (p < .05). Twelve fatty acids were identified in oil samples, while their percentage contents were not significantly different during the refining process. Contents of tocopherol and total phenols were removed by 23.5% and 79.4%, respectively, after refining. The refining process resulted in losses of campesterol and β-sitosterol of approximately 16.0% and 13.8%, respectively. Moreover, squalene was detected in evening primrose oil for the first time, and its content decreased by 20.2% during the refining process. Furthermore, during the whole refining process, the DPPH free radical-scavenging ability of EPO decreased by 10.6%, and the FRAP reducing capacity dropped by 56%, meanwhile, its antioxidant stability was affected. How to cite this article: Pan F, Li Y, Luo X, et al. Effect of the chemical refining process on composition and oxidative stability of evening primrose oil.

This study developed novel oral delivery systems for the encapsulation, protection, and controlled release of hydrophobic and hydrophilic bioactive compounds based on l-arginine- or l-lysine-functionalized chitosan–casein nanoparticles.