No abstract

mediated isothermal amplification (RT-LAMP). These techniques were also validated using samples from ZIKV infected patients and mosquitoes.RESULTS: RT-LAMP could specifically detect ZIKV in ZIKV positive samples. This could be done in under 30 minutes and did not require timely RNA extraction from the urine, unlike real time PCR.CONCLUSIONS: Here we describe a technique by which ZIKV can be rapidly detected in a non-invasive urine sample. This may allow for easy monitoring of potentially exposed individuals, especially pregnant women, couples wanting to conceive, or individuals with suspicious symptoms.Source of Funding: This study was funded in part by the Maureen and Ronald Hirsch family philanthropic contribution.