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Rhodopseudomonas palustris CGA009 is a metabolically robust microbe that can utilize lignin breakdown products to produce polyhydroxyalkanoates (PHAs), biopolymers with the potential to replace conventional plastics. Our recent efforts suggest PHA granule formation is a limiting factor for maximum production of the bioplastic poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) by R. palustris. The Phap1 phasin ( phaP1 ) from the PHB-producing model bacterium Cupriavidus necator H16 was expressed in R. palustris with the aim of overproducing PHBV from the lignin breakdown product p- coumarate by fostering smaller and more abundant granules. Expression of phaP1 yielded PHBV production from R. palustris aerobically (0.7 g/L), which does not occur in the wild-type strain, and led to a significantly higher PHBV titer than wild-type anaerobic production (0.41 g/L). The 3HV fractions were also significantly increased under both anaerobic and aerobic conditions, which boosts thermomechanical properties and potential for application. Thus, heterologous phasin expression in R. palustris provides flexibility for industrial processing and could foster compositional changes in copolymers with better thermomechanical properties compared to PHB alone.

Highlights Teamwork data from engineering capstone courses were analyzed to detect impacts of emergency remote teaching. The Comprehensive Assessment for Team-Member Effectiveness (CATME) data was analyzed via statistical modeling. Qualitative data attained from student responses were analyzed for patterns. Students found the lack of team camaraderie even more challenging than limitations on testing designs. This study offers avenues for developing engineering students’ teamwork skills in remote settings. Abstract. The onset of the global pandemic forced universities to rapidly shift to emergency remote teaching (ERT), which could cause even more perturbations for engineering courses with a hands-on, project-oriented focus. Thus, the purpose of this project was to gain a data-driven appreciation of how teamwork performance was impacted for engineering students in this environment and recommend focus areas for instructional designers. The Comprehensive Assessment for Team-Member Effectiveness (CATME) tool was used to assess different aspects of teamwork performance for 108 students in an undergraduate engineering capstone course during an in-person course offered in 2019-2020 (pre-pandemic) and an ERT course offered in 2020-2021 at a major Midwestern university. The classes were divided into teams for their capstone projects using the CATME Team-Maker tool. Students were asked to rate their teammates at the beginning, middle, and end of the course across five CATME dimensions: (1) Contribution to Team’s Work, (2) Interacting with Teammates, (3) Keeping the Team on Track, (4) Expecting Quality, and (5) Having Relevant Knowledge, Skills, and Abilities (KSAs). Statistical modeling was implemented to decipher how ratings differed throughout the year in each course as well as to identify specific CATME areas that varied between the in-person and ERT courses. A qualitative assessment was also implemented for the ERT course based on student responses to a prompt that asked them to comment on how the pandemic impacted their personal and team performance. Results revealed that engineering students showed a significant reduction in three categories in the ERT course compared to in-person: Contributing to Team’s Work, Expecting Quality, and Having Relevant KSAs. Interestingly, these three categories deal largely with student motivation toward team efforts, which was echoed in the qualitative assessment. The majority of alarming comments made by students were regarding not being able to build camaraderie with their teammates in the ERT environment. It was surprising to find that engineering students found this lack of team camaraderie even more challenging than the limitations on testing their designs. Thus, more data-driven analyses are necessary to examine which methods and technologies are ideal for teleworking project-based courses in terms of facilitating team bonding, helping teams brainstorm, and fostering more engaging communication between team members. Future efforts should build upon this exploratory study and employ a larger sample size so that results can be generalized to all courses and also provide insight into disparities between subpopulations such as race, gender, or international status.